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Article

tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci.

Nucleic acids research - 1 Dec 2017

Ott Alina, Liu Sanzhen, Schnable James C, Yeh Cheng-Ting 'Eddy', Wang Kai-Sin, Schnable Patrick S

Abstract excerpt

Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than (double-stranded) adaptors are ligated. This strategy ensures...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Polymorphism, Single Nucleotide

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Source

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Identifiers

PMID: 29036322
DOI: 10.1093/nar/gkx853
PMCID: PMC5716196

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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