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Article

Control of the pump cycle in bacteriorhodopsin: mechanisms elucidated by solid-state NMR of the D85N mutant.

Biophysical journal - 1 Feb 2002

Hatcher Mary E, Hu Jingui G, Belenky Marina, Verdegem Peter, Lugtenburg Johan, Griffin Robert G, Herzfeld Judith

Abstract excerpt

By varying the pH, the D85N mutant of bacteriorhodopsin provides models for several photocycle intermediates of the wild-type protein in which D85 is protonated. At pH 10.8, NMR spectra of [zeta-(15)N]lys-, [12-(13)C]retinal-, and [14,15-(13)C]retinal-labeled D85N samples indicate a deprotonated, 13-cis,15-anti chromophore. On the other hand, at neutral pH, the NMR spectra of D85N show a mixture of protonated...

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Identifiers

PMID: 11806941
DOI: 10.1016/S0006-3495(02)75461-1
PMCID: PMC1301908

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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