Article
On the heterogeneity of the M population in the photocycle of bacteriorhodopsin.
Biochemistry - 13 Dec 1994
Friedman N, Gat Y, Sheves M, Ottolenghi M
Abstract excerpt
The M stage in the photocycle of bacteriorhodopsin (bR), a key step in its light-induced proton pump mechanism, is studied in water/glycerol suspensions over the temperature range between 20 and -60 degrees C. The biexponential decay of M is analyzed for wild-type (WT) bR and for its D96N, Y185F, and D115N mutants, at various pH values, according to the scheme: bR-->(hv) L-->M<-->(k1, k-1) N-->(k2) bR. The...
Topics
- Bacteriorhodopsins
- Halobacterium
- Hydrogen-Ion Concentration
- Kinetics
- Light
- Models, Chemical
- Mutation
- Proton Pumps
- Thermodynamics