Article
Characterization of photo-intermediates in the photo-reaction pathways of a bacteriorhodopsin Y185F mutant using in situ photo-irradiation solid-state NMR spectroscopy.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 26 Sept 2015
Oshima Kyosuke, Shigeta Arisu, Makino Yoshiteru, Kawamura Izuru, Okitsu Takashi, Wada Akimori, Tuzi Satoru, Iwasa Tatsuo, Naito Akira
Abstract excerpt
Photo-reaction pathways of a bacteriorhodopsin Y185F mutant were examined using in situ photo-irradiation solid-state NMR spectroscopy. (13)C CP MAS NMR spectra were recorded at -40 °C in the dark (D1), under irradiation with 520 nm light (L1), subsequently in the dark (D2), and again under irradiation with 520 nm light (L2). In the process from D1 to L1, the 13-cis, 15-syn (CS; bR548) state changed to a CS*-...
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