Article
Suppressors of transcriptional transgenic silencing in Chlamydomonas are sensitive to DNA-damaging agents and reactivate transposable elements.
Proceedings of the National Academy of Sciences of the United States of America - 22 Jan 2002
Jeong Br Byeong-ryool, Wu-Scharf Dancia, Zhang Chaomei, Cerutti Heriberto
Abstract excerpt
In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of single-copy transgenes, transcriptional silencing takes place without detectable cytosine methylation of the introduced DNA. We have isolated two mutant strains, Mut-9 and Mut-11, that reactivate expression of a...
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