Article
An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas.
Nature communications - 8 Dec 2020
Neupert Juliane, Gallaher Sean D, Lu Yinghong, Strenkert Daniela, Segal Na'ama, Barahimipour Rouhollah, Fitz-Gibbon Sorel T, Schroda Michael, Merchant Sabeeha S, Bock Ralph
Abstract excerpt
Silencing of exogenous DNA can make transgene expression very inefficient. Genetic screens in the model alga Chlamydomonas have demonstrated that transgene silencing can be overcome by mutations in unknown gene(s), thus producing algal strains that stably express foreign genes to high levels. Here, we show that the silencing mechanism specifically acts on transgenic DNA. Once a permissive chromatin structure has...
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