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Article

Truncated green fluorescent protein mutants and their expression in Aplysia neurons.

Brain research bulletin - 1 Sept 1998

Kim H K, Kaang B K

Abstract excerpt

We determined in detail the primary structure requirements for green fluorescence of the jellyfish green fluorescent protein (GFP) and of its improved mutants (S65T and 1167T). We performed a deletion mapping in combination with fluorescence-activated cell sorting (FACS) and spectrofluorometry. This showed that deletion of up to nine amino acids at the C-terminal end of GFP had no deleterious effect on the...

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PMID: 9766387
DOI: 10.1016/s0361-9230(98)00020-3

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