Article
Truncated green fluorescent protein mutants and their expression in Aplysia neurons.
Brain research bulletin - 1 Sept 1998
Kim H K, Kaang B K
Abstract excerpt
We determined in detail the primary structure requirements for green fluorescence of the jellyfish green fluorescent protein (GFP) and of its improved mutants (S65T and 1167T). We performed a deletion mapping in combination with fluorescence-activated cell sorting (FACS) and spectrofluorometry. T...
Topics
- Animals
- Aplysia
- Cell Separation
- Cells, Cultured
- Chromosome Deletion
- Feasibility Studies
- Flow Cytometry
- Gene Expression Regulation
- Genetic Markers
- Genetic Variation
- Green Fluorescent Proteins
- Indicators and Reagents
- Luminescent Proteins
- Mutation
- Nerve Tissue Proteins
- Neurons
- Spectrometry, Fluorescence
