Structure-function analysis of T4 RNA ligase 2.

doi:10.1074/jbc.M300817200

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Article

Structure-function analysis of T4 RNA ligase 2.

The Journal of biological chemistry - 16 May 2003

Yin Shenmin, Ho C Kiong, Shuman Stewart

Abstract excerpt

Bacteriophage T4 RNA ligase 2 (Rnl2) exemplifies a polynucleotide ligase family that includes the trypanosome RNA-editing ligases and putative RNA ligases encoded by eukaryotic viruses and archaea. Here we analyzed 12 individual amino acids of Rnl2 that were identified by alanine scanning as essential for strand joining. We determined structure-activity relationships via conservative substitutions and examined...

Topics

Adenosine Triphosphate
Alanine
Amino Acid Sequence
Binding Sites
Dose-Response Relationship, Drug
Lysine
Molecular Sequence Data
Mutagenesis
Mutagenesis, Site-Directed
Mutation
Nucleic Acid Conformation
Polynucleotide Ligases
RNA
RNA Ligase (ATP)
Recombinant Proteins
Sequence Homology, Amino Acid
Structure-Activity Relationship
Viral Proteins

Source

Open in PubMed

Identifiers

PMID: 12611899
DOI: 10.1074/jbc.M300817200

Provenance

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