Article
Nick recognition by DNA ligases.
Journal of molecular biology - 11 Feb 2000
Doherty A J, Dafforn T R
Abstract excerpt
Phage T7 DNA ligase seals nicked DNA substrates and is a representative member of the ATP-dependent class of DNA ligases. Although the catalytic mechanism of DNA ligases has been delineated, little is known about the nature of nick recognition by these enzymes. Here, we show that T7 ligase discriminates, at the nick-binding step, between nicks containing either a 5'-phosphate or a 5'-OH. T7 ligase binds...
Topics
- Amino Acid Sequence
- Bacteriophage T7
- Base Sequence
- Binding Sites
- Catalysis
- Conserved Sequence
- DNA
- DNA Damage
- DNA Footprinting
- DNA Ligases
- Lysine
- Models, Molecular
- Molecular Conformation
- Mutation
- Oligodeoxyribonucleotides
- Phosphates
- Protein Binding
- Substrate Specificity