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Article

Nick sensing by vaccinia virus DNA ligase requires a 5' phosphate at the nick and occupancy of the adenylate binding site on the enzyme.

Journal of virology - 1 Dec 1997

Sekiguchi J, Shuman S

Abstract excerpt

Vaccinia virus DNA ligase has an intrinsic nick-sensing function. The enzyme discriminates at the substrate binding step between a DNA containing a 5' phosphate and a DNA containing a 5' hydroxyl at the nick. Further insights into nick recognition and catalysis emerge from studies of the active-s...

Topics

Adenosine Monophosphate
Binding Sites
Catalysis
DNA Ligases
DNA Nucleotidyltransferases
DNA Topoisomerases, Type I
Mutation
Phosphates
Vaccinia virus

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 9371633
DOI: 10.1128/JVI.71.12.9679-9684.1997
PMCID: PMC230277

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9371633-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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