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Article

Nick sensing by vaccinia virus DNA ligase requires a 5' phosphate at the nick and occupancy of the adenylate binding site on the enzyme.

Journal of virology - 1 Dec 1997

Sekiguchi J, Shuman S

Abstract excerpt

Vaccinia virus DNA ligase has an intrinsic nick-sensing function. The enzyme discriminates at the substrate binding step between a DNA containing a 5' phosphate and a DNA containing a 5' hydroxyl at the nick. Further insights into nick recognition and catalysis emerge from studies of the active-site mutant K231A, which is unable to form the covalent ligase-adenylate intermediate and hence cannot activate a nicked...

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Identifiers

PMID: 9371633
DOI: 10.1128/JVI.71.12.9679-9684.1997
PMCID: PMC230277

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