Article
Outer membrane composition of a lipopolysaccharide-deficient Neisseria meningitidis mutant.
The EMBO journal - 17 Dec 2001
Steeghs L, de Cock H, Evers E, Zomer B, Tommassen J, van der Ley P
Abstract excerpt
In the pathogen Neisseria meningitidis, a completely lipopolysaccharide (LPS)-deficient but viable mutant can be obtained by insertional inactivation of the lpxA gene, encoding UDP-GlcNAc acyltransferase required for the first step of lipid A biosynthesis. To study how outer membrane structure and biogenesis are affected by the absence of this normally major component, inner and outer membranes were separated and...
Topics
- Base Sequence
- Cell Membrane
- DNA Primers
- Lipopolysaccharides
- Membrane Lipids
- Mutation
- Neisseria meningitidis
- Permeability
- Phospholipids