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Article

High throughput assay for cytochrome P450 BM3 for screening libraries of substrates and combinatorial mutants.

Biosensors & bioelectronics - 1 Jan 2002

Tsotsou Georgia Eleni, Cass Anthony Edward George, Gilardi Gianfranco

Abstract excerpt

A rapid method for identifying compounds that are potential substrates for the drug metabolising enzyme cytochrome P450 is described. The strategy is based on the detection of a degradation product of NAD(P)H oxidation during substrate turnover by the enzyme expressed in Escherichia coli cells spontaneously lysed under the experimental conditions. The performance of the method has been tested on two known...

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PMID: 11742743
DOI: 10.1016/s0956-5663(01)00285-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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