Transduction-mediated transfer of unmarked deletion and point mutations through use of counterselectable suicide vectors.

Abstract excerpt

A challenge in strain construction is that unmarked deletion and nucleotide substitution alleles generally do not confer selectable phenotypes. We describe here a rapid and efficient strategy for transferring such alleles via generalized transduction. The desired allele is first constructed and introduced into the chromosome by conventional allelic-exchange methods. The suicide vector containing the same allele...

Topics

• Bacteriophage P22
• Genetic Vectors
• Mutation
• Point Mutation
• Salmonella typhimurium
• Selection, Genetic
• Sequence Deletion
• Transduction, Genetic