Article
Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose
1 Oct 2001
Abstract excerpt
The 9E10 antibody epitope (EQKLISEEDL) derives from a protein sequence in the human proto-oncogen p62(c-myc) and is widely used as a protein fusion tag. This myc-tag is a powerful tool in protein localization, immunochemistry, ELISA or protein purification. Here, we characterize the myc-tag epitope by substitutional analysis and length variation using peptide spot synthesis on cellulose. The key amino acids of...
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