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Detecting low level sequence variants in recombinant monoclonal antibodies

1 May 2010

Abstract excerpt

A systematic analytical approach combining tryptic and chymotryptic peptide mapping with a Mascot Error Tolerant Search (ETS) has been developed to detect and identify low level protein sequence variants, i.e., amino acid substitutions, in recombinant monoclonal antibodies. The reversed-phase HPLC separation with ultraviolet (UV) detection and mass spectral acquisition parameters of the peptide mapping methods...

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Share to Monoclonal and Polyclonal Antibodies Research
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PMID: 20400866
DOI: 10.4161/mabs.2.3.11718

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