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C‐terminal lysine variants in fully human monoclonal antibodies: Investigation of test methods and possible causes

4 Mar 2008

Abstract excerpt

The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were evaluated for their ability to effectively approximate relative percentages of lysine variants. A...

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PMID: 18553400
DOI: 10.1002/bit.21855

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