Article
Evidence for coupling of membrane targeting and function of the signal recognition particle (SRP) receptor FtsY.
EMBO reports - 1 Nov 2001
Herskovits A A, Seluanov A, Rajsbaum R, ten Hagen-Jongman C M, Henrichs T, Bochkareva E S, Phillips G J, Probst F J, Nakae T, Ehrmann M, Luirink J, Bibi E
Abstract excerpt
Recent studies have indicated that FtsY, the signal recognition particle receptor of Escherichia coli, plays a central role in membrane protein biogenesis. For proper function, FtsY must be targeted to the membrane, but its membrane-targeting pathway is unknown. We investigated the relationship between targeting and function of FtsY in vivo, by separating its catalytic domain (NG) from its putative targeting...
Topics
- Bacterial Proteins
- Blotting, Western
- Catalytic Domain
- Cell Fractionation
- Cell Membrane
- Escherichia coli
- Models, Biological
- Mutation
- Plasmids
- Protein Binding
- Protein Biosynthesis
- Protein Structure, Tertiary
- Receptors, Cytoplasmic and Nuclear