Neurospora crassa tox-1 Gene Encodes a pH- and Temperature-Tolerant Mini-Cellulase.

doi:10.1021/acs.jafc.6b00043

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Article

Neurospora crassa tox-1 Gene Encodes a pH- and Temperature-Tolerant Mini-Cellulase.

Journal of agricultural and food chemistry - 15 Jun 2016

Xiao Yue, Zhang Qiongsi, Luo Yiquan, Zhang Ying, Luo Xi, Wang Yuchuan, Cao Weiguo, Pinto Vito De, Liu Qiuyun, Li Gang

Abstract excerpt

Cellulases that endure extreme conditions are essential in various industrial sectors. This study reports a mini-cellulase gene tox-1 from Neurospora crassa. The gene tox-1 was cloned in Escherichia coli after chimerization with the YebF gene and substitutions of certain isoleucine and valine with leucine residues. The yeast transformants could grow on rice straw-agar medium. The 44-amino acid peptide and its two...

Topics

Cellulase
Circular Dichroism
Cloning, Molecular
Escherichia coli
Fungal Proteins
Hydrogen-Ion Concentration
Kinetics
Mutation
Neurospora crassa
Recombinant Proteins
Saccharomyces cerevisiae
Solvents
Substrate Specificity
Surface-Active Agents

Source

Open in PubMed

Identifiers

PMID: 27229865
DOI: 10.1021/acs.jafc.6b00043

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