Article
Rapid and precise epitope mapping of monoclonal antibodies against Plasmodium falciparum AMA1 by combined phage display of fragments and random peptides.
Protein engineering - 1 Sept 2001
Coley A M, Campanale N V, Casey J L, Hodder A N, Crewther P E, Anders R F, Tilley L M, Foley M
Abstract excerpt
We describe an approach for the rapid mapping of epitopes within a malaria antigen using a combination of phage display techniques. Phage display of antigen fragments identifies the location of the epitopes, then random peptide libraries displayed on phage are employed to identify accurately amino acids involved in the epitope. Finally, phage display of mutant fragments confirms the role of each residue in the...
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