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Article

Probing tertiary structure of proteins using single Trp mutations with circular dichroism at low temperature.

The journal of physical chemistry. B - 30 Jan 2014

Gasymov Oktay K, Abduragimov Adil R, Glasgow Ben J

Abstract excerpt

Trp is the most spectroscopically informative aromatic amino acid of proteins. However, the near-UV circular dichroism (CD) spectrum of Trp is complicated because the intensity and sign of (1)La and (1)Lb bands vary independently. To resolve vibronic structure and gain site-specific information from complex spectra, deconvolution was combined with cooling and site-directed tryptophan substitution. Low temperature...

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Identifiers

PMID: 24404774
DOI: 10.1021/jp4120145
PMCID: PMC3983331

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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