Article
The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme.
Molecular and cellular biology - 1 Dec 2001
Hon T, Lee H C, Hach A, Johnson J L, Craig E A, Erdjument-Bromage H, Tempst P, Zhang L
Abstract excerpt
In Saccharomyces cerevisiae, heme directly mediates the effects of oxygen on transcription through the heme activator protein Hap1. In the absence of heme, Hap1 is bound by at least four cellular proteins, including Hsp90 and Ydj1, forming a higher-order complex, termed HMC, and its activity is repressed. Here we purified the HMC and showed by mass spectrometry that two previously unidentified major components of...
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