Article
Expression and crystallization of an N-terminally activated form of the Bacillus thuringiensis Cry1Ca toxin.
Current microbiology - 1 Nov 2001
Kouskoura T, Tickner C, Crickmore N
Abstract excerpt
When the active form of the Bacillus thuringiensis delta-endotoxin Cry1Ca was expressed in E. coli severe growth retardation was observed. The absence of a short peptide from the N-terminus of the protoxin was responsible for this effect. The introduction of a mutation at an amino acid previously reported as being involved in the initial stages of pore formation within the natural insect target partially...
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