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Article

Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC delta-endotoxin affects insecticidal specificity.

The Biochemical journal - 1 Sept 1994

Smith G P, Ellar D J

Abstract excerpt

Site-directed mutagenesis was used to determine the role of two surface-exposed loops (Gly-317-Phe-320 and Gln-374-Pro-377) in the insecticidal specificity of the Bacillus thuringiensis CryIC delta-endotoxin. Mutant toxins were generated by PCR using degenerate oligonucleotide primers, and expressed in Escherichia coli. More than 50 mutant toxins were screened for toxicity to the lepidopteran Spodoptera...

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Identifiers

PMID: 8093015
DOI: 10.1042/bj3020611
PMCID: PMC1137271

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