Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC delta-endotoxin affects insecticidal specificity.

doi:10.1042/bj3020611

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC delta-endotoxin affects insecticidal specificity.

The Biochemical journal - 1 Sept 1994

Smith G P, Ellar D J

Abstract excerpt

Site-directed mutagenesis was used to determine the role of two surface-exposed loops (Gly-317-Phe-320 and Gln-374-Pro-377) in the insecticidal specificity of the Bacillus thuringiensis CryIC delta-endotoxin. Mutant toxins were generated by PCR using degenerate oligonucleotide primers, and expressed in Escherichia coli. More than 50 mutant toxins were screened for toxicity to the lepidopteran Spodoptera...

Topics

Aedes
Amino Acid Sequence
Animals
Bacillus thuringiensis Toxins
Bacterial Proteins
Bacterial Toxins
Base Sequence
Cell Line
Electrophoresis, Polyacrylamide Gel
Endotoxins
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hemolysin Proteins

Insecticides

Molecular Sequence Data

Moths

Mutagenesis, Site-Directed

Mutation

Plasmids

Source

Open in PubMed Open full text

Identifiers

PMID: 8093015
DOI: 10.1042/bj3020611
PMCID: PMC1137271

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8093015-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC delta-endotoxin affects insecticidal specificity.PMID 8093015Read ↗

Analysis of mutations in the pore-forming region essential for insecticidal activity of a Bacillus thuringiensis delta-endotoxin.Semantic proximity Read ↗

Enhanced toxicity of Bacillus thuringiensis Cry3A delta-endotoxin in coleopterans by mutagenesis in a receptor binding loop.Semantic proximity Read ↗

Functional significance of loops in the receptor binding domain of Bacillus thuringiensis CryIIIA delta-endotoxin.Semantic proximity Read ↗

Directed mutagenesis of selected regions of a Bacillus thuringiensis entomocidal protein.Semantic proximity Read ↗

Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action.Semantic proximity Read ↗

Effects on larvicidal activity of single proline substitutions in alpha3 or alpha4 of the Bacillus thuringiensis Cry4B toxin.Semantic proximity Read ↗

Essential role of amino acids in αD-β4 loop of a Bacillus thuringiensis Cyt2Aa2 toxin in binding and complex formation on lipid membrane.Semantic proximity Read ↗

N546 in beta18-beta19 loop is important for binding and toxicity of the Bacillus thuringiensis Cry1Ac toxin.Semantic proximity Read ↗

Specific mutations within the alpha4-alpha5 loop of the Bacillus thuringiensis Cry4B toxin reveal a crucial role for Asn-166 and Tyr-170.Semantic proximity Read ↗

Structural and functional studies of alpha-helix 5 region from Bacillus thuringiensis Cry1Ab delta-endotoxin.Semantic proximity Read ↗

Pesticidal and receptor binding properties of Bacillus thuringiensis Cry1Ab and Cry1Ac delta-endotoxin mutants to Pectinophora gossypiella and Helicoverpa zea.Semantic proximity Read ↗

Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.