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Article

Effects on larvicidal activity of single proline substitutions in alpha3 or alpha4 of the Bacillus thuringiensis Cry4B toxin.

Biochemistry and molecular biology international - 1 Apr 1998

Uawithya P, Tuntitippawan T, Katzenmeier G, Panyim S, Angsuthanasombat C

Abstract excerpt

The possible role of alpha-helices 3 and 4 in toxicity of the dipteran-active Bacillus thuringiensis Cry4B delta-endotoxin was investigated by employing proline substitutions via site-directed mutagenesis. Similar to the wild-type Cry4B, the mutant toxins were over-expressed in Escherichia coli as cytoplasmic inclusions and were structurally stable upon solubilization and trypsin activation. The substitution of...

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Identifiers

PMID: 9584996
DOI: 10.1080/15216549800201872

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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