Article
Simulating pseudogene evolution in vitro: determining the true number of mutations in a lineage.
Proceedings of the National Academy of Sciences of the United States of America - 6 Nov 2001
Vartanian J P, Henry M, Wain-Hobson S
Abstract excerpt
Hypermutagenic PCR has been used to simulate pseudogene evolution of the Escherichia coli R67 dihydrofolate reductase gene. Each time the most divergent clone was used as template for another round of hypermutagenesis. After six rounds, with an average mutation rate of 0.05 per base per round, up to a 46% nucleic acid sequence variation was achieved. For a few clones the protein information content could be...
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