Article
Predicting evolution by in vitro evolution requires determining evolutionary pathways.
Antimicrobial agents and chemotherapy - 1 Sept 2002
Hall Barry G
Abstract excerpt
In an early example of DNA shuffling, Stemmer (W. P. C. Stemmer, Nature 370:389-390, 1994) demonstrated a dramatic improvement in the activity of the TEM-1 beta-lactamase toward cefotaxime as the consequence of six amino acid substitutions. It has been pointed out (B. G. Hall, FEMS Microbiol. Lett. 178:1-6, 1999; M. C. Orencia, J. S. Yoon, J. E. Ness, W. P. Stemmer, and R. C. Stevens, Nat. Struct. Biol....
Topics
- Alleles
- Amino Acid Substitution
- Biological Evolution
- DNA Shuffling
- DNA, Bacterial
- Drug Resistance, Microbial
- Mutation
- beta-Lactamases