Article
Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990
Hermes J D, Blacklow S C, Knowles J R
Abstract excerpt
How easy is it to improve the catalytic power of an enzyme? To address this question, the gene encoding a sluggish mutant triose-phosphate isomerase (D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1) has been subjected to random mutagenesis over its whole length by using "spiked" oligonuc...
Topics
- Aspartic Acid
- Carbohydrate Epimerases
- Escherichia coli
- Genes, Bacterial
- Glutamates
- Glutamic Acid
- Kinetics
- Models, Molecular
- Mutation
- Oligonucleotide Probes
- Plasmids
- Protein Conformation
- Triose-Phosphate Isomerase