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Article

Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme.

Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990

Hermes J D, Blacklow S C, Knowles J R

Abstract excerpt

How easy is it to improve the catalytic power of an enzyme? To address this question, the gene encoding a sluggish mutant triose-phosphate isomerase (D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1) has been subjected to random mutagenesis over its whole length by using "spiked" oligonucleotide primers. Transformation of an isomerase-minus strain of Escherichia coli was followed by selection of those...

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Identifiers

PMID: 1967829
DOI: 10.1073/pnas.87.2.696
PMCID: PMC53332

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Source receipt: pubmed, retrieved 7 Sept 2026.

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