Article
The crystal structure and catalytic mechanism of hydroxynitrile lyase from passion fruit, Passiflora edulis.
The FEBS journal - 1 Jan 2018
Motojima Fumihiro, Nuylert Aem, Asano Yasuhisa
Abstract excerpt
Hydroxynitrile lyases (HNLs) are enzymes used in the synthesis of chiral cyanohydrins. The HNL from Passiflora edulis (PeHNL) is R-selective and is the smallest HNL known to date. The crystal structures of PeHNL and its C-terminal peptide depleted derivative were determined by molecular replacement method using the template structure of a heat stable protein, SP1, from Populus tremula at 2.8 and 1.8 Å resolution,...
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