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Article

Insight into autoproteolytic activation from the structure of cephalosporin acylase: a protein with two proteolytic chemistries.

Proceedings of the National Academy of Sciences of the United States of America - 7 Feb 2006

Kim Jin Kwang, Yang In Seok, Shin Hye Jeong, Cho Ki Joon, Ryu Eui Kyung, Kim Sun Hwa, Park Sung Soo, Kim Kyung Hyun

Abstract excerpt

Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through sequential primary and secondary autoproteolytic reactions with the release of a pro segment. We have determined crystal structures of four CA mutants. Two mutants are trapped after the primary cleavage, and the other two undergo secondary cleavage slowly. These structures provide a look at pro-segment...

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Identifiers

PMID: 16446446
DOI: 10.1073/pnas.0507862103
PMCID: PMC1413634

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