Article
Acetobacter turbidans alpha-amino acid ester hydrolase: how a single mutation improves an antibiotic-producing enzyme.
The Journal of biological chemistry - 3 Mar 2006
Barends Thomas R M, Polderman-Tijmes Jolanda J, Jekel Peter A, Williams Christopher, Wybenga Gjalt, Janssen Dick B, Dijkstra Bauke W
Abstract excerpt
The alpha-amino acid ester hydrolase (AEH) from Acetobacter turbidans is a bacterial enzyme catalyzing the hydrolysis and synthesis of beta-lactam antibiotics. The crystal structures of the native enzyme, both unliganded and in complex with the hydrolysis product D-phenylglycine are reported, as well as the structures of an inactive mutant (S205A) complexed with the substrate ampicillin, and an active site mutant...
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