Article
Using PAC nested deletions to order contigs and microsatellite markers at the high repetitive sequence containing Npr3 gene locus.
Gene - 5 Sept 2001
Gilmore R C, Baker J, Dempsey S, Marchan R, Corprew R N, Byrd G, Maeda N, Smithies O, Bukoski R D, Harewood K R, Chatterjee P K
Abstract excerpt
Highly polymorphic di- and tetranucleotide repeats in and around Npr3, a potential candidate gene for hypertension, have been identified using a novel approach. Because this chromosomal site is rich in repetitive DNA and difficult to sequence, P1 artificial chromosomes were retrofitted with a loxP transposon to map the gene sequence within a clone using a series of nested deletions. Sequences from ends of...
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