Article
Direct sequencing of bacterial and P1 artificial chromosome-nested deletions for identifying position-specific single-nucleotide polymorphisms.
Proceedings of the National Academy of Sciences of the United States of America - 9 Nov 1999
Chatterjee P K, Yarnall D P, Haneline S A, Godlevski M M, Thornber S J, Robinson P S, Davies H E, White N J, Riley J H, Shepherd N S
Abstract excerpt
A loxP-transposon retrofitting strategy for generating large nested deletions from one end of the insert DNA in bacterial artificial chromosomes and P1 artificial chromosomes was described recently [Chatterjee, P. K. & Coren, J. S. (1997) Nucleic Acids Res. 25, 2205-2212]. In this report, we combine this procedure with direct sequencing of nested-deletion templates by using primers located in the transposon end...
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