Article
Ethanol inhibition of N-methyl-D-aspartate receptors is reduced by site-directed mutagenesis of a transmembrane domain phenylalanine residue.
The Journal of biological chemistry - 30 Nov 2001
Ronald K M, Mirshahi T, Woodward J J
Abstract excerpt
N-Methyl-D-aspartate (NMDA) receptors (NRs) are ionotropic receptors activated by glutamate and the co-agonist glycine. Ethanol inhibits NMDA receptor function, although its site of action is undefined. We hypothesized that ethanol acts at specific amino acids contained within the transmembrane (TM) domains of the receptor. In this study, NR1 and NR2A subunits were altered by mutagenesis and tested for...
Topics
- Amino Acid Sequence
- Animals
- Binding, Competitive
- Cell Line
- Dose-Response Relationship, Drug
- Ethanol
- Excitatory Amino Acid Antagonists
- Glutamic Acid
- Glycine
- Humans
- Kynurenic Acid
- Molecular Sequence Data
- Mutagenesis, Site-Directed