Article
Cloning and characterization of a lipid-activated CTP:phosphocholine cytidylyltransferase from Caenorhabditis elegans: identification of a 21-residue segment critical for lipid activation.
Biochimica et biophysica acta - 28 Sept 2001
Friesen J A, Liu M F, Kent C
Abstract excerpt
The genome of the nematode Caenorhabditis elegans contains several genes that appear to encode proteins similar to CTP:phosphocholine cytidylyltransferase (CCT). We have isolated a 1044-nucleotide cDNA clone from a C. elegans cDNA library that encodes the 347-amino acid version of CCT that is most similar to previously-identified CCTs. Native and His-tagged forms were expressed and purified using a baculovirus...
Topics
- Amino Acid Sequence
- Animals
- Caenorhabditis elegans
- Choline-Phosphate Cytidylyltransferase
- DNA, Complementary
- Diglycerides
- Enzyme Activation
- Isoenzymes
- Kinetics
- Lipids
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oleic Acid