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Identification of critical residues of choline kinase A2 from Caenorhabditis elegans.

The Journal of biological chemistry - 23 Apr 2004

Yuan Chong, Kent Claudia

Abstract excerpt

Choline kinase catalyzes the phosphorylation of choline by ATP, the first committed step in the CDP-choline pathway for phosphatidylcholine biosynthesis. To begin to elucidate the mechanism of catalysis by this enzyme, choline kinase A-2 from Caenorhabditis elegans was analyzed by systematic mutagenesis of highly conserved residues followed by analysis of kinetic and structural parameters. Specifically, mutants...

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PMID: 14960577
DOI: 10.1074/jbc.M401382200

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