Article
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Molecular cell - 1 Aug 2001
Suka N, Suka Y, Carmen A A, Wu J, Grunstein M
Abstract excerpt
We have developed a highly specific antibody set for acetylation sites in yeast histones H4 (K5, K8, K12, and K16); H3 (K9, K14, K18, K23, and K27); H2A (K7); and H2B (K11 and K16). Since ELISA does not assure antibody specificity in chromatin immunoprecipitation, we have employed additional screens against the respective histone mutations. We now show that telomeric and silent mating locus heterochromatin is...
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