Article
Positive selection of mutations leading to loss or reduction of transcriptional activity of PrfA, the central regulator of Listeria monocytogenes virulence.
Journal of bacteriology - 1 Oct 2001
Herler M, Bubert A, Goetz M, Vega Y, Vazquez-Boland J A, Goebel W
Abstract excerpt
Transcription factor PrfA controls the expression of virulence genes essential for Listeria monocytogenes pathogenesis. To gain insight into the structure-function relationship of PrfA, we devised a positive-selection system to isolate mutations reducing or abolishing transcriptional activity. The system is based on the observation that the listerial iap gene, encoding the p60 protein, is lethal if overexpressed...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Bacterial Toxins
- Gene Expression Regulation, Bacterial
- Heat-Shock Proteins
- Hemolysin Proteins
- Listeria monocytogenes
- Molecular Sequence Data
- Mutation