Article
A naturally occurring mutation K220T in the pleiotropic activator PrfA of Listeria monocytogenes results in a loss of virulence due to decreasing DNA-binding affinity.
Microbiology (Reading, England) - 1 Apr 2007
Velge P, Herler M, Johansson J, Roche S M, Témoin S, Fedorov A A, Gracieux P, Almo S C, Goebel W, Cossart P
Abstract excerpt
The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low-virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thus no virulence. This substitution inactivated PrfA, since expression of the PrfAK220T mutant gene in an EGDDeltaprfA...
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