Article
Characterization of a Saccharomyces cerevisiae mutant with oversecretion phenotype.
Applied microbiology and biotechnology - 1 Jun 2001
Wang B D, Chen D C, Kuo T T
Abstract excerpt
An oversecreting mutant of Saccharomyces cerevisiae was obtained from about 400 meiotic segregants derived from thediploid cells made by crossing the HBsAg-induced mutant NI-C with the wild-type strain Sey6211. When transformed with a plasmid containing mouse alpha-amylase cDNA, the mutant (NI-C-D4) exhibited an increased capacity (up to 13-fold) for the secretion of mouse alpha-amylase, higher than the parental...
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