Article
Optimization of single-stranded conformation polymorphism (SSCP) analysis for screening for the estrogen receptor-alpha gene polymorphism P325P.
Clinical chemistry and laboratory medicine - 1 Jul 2001
Bitenc D, Marc J
Abstract excerpt
Since there are no theoretical models for single-stranded conformation polymorphism (SSCP) analysis, conditions for detecting specific mutation must be found experimentally. Previously, a time-consuming (22 hours) SSCP method was used for the detection of polymorphism in codon 325 (CCC to CCG; P325P) in exon 4 of estrogen receptor-alpha gene. The aim of our work was to study different gel loading buffers,...
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