Article
Role of medium- and long-range interactions to the stability of the mutants of T4 lysozyme.
Preparative biochemistry & biotechnology - 1 Aug 2001
Gromiha M M, Thangakani A M
Abstract excerpt
Inter-residue interactions play an important role to the folding and stability of protein molecules. In this work, we analyze the role of medium- and long-range interactions to the stability of T4 lysozyme mutants. We found that, in buried mutations, the increase in long-range contacts upon mutations destabilizes the protein, whereas, in surface mutations, the increase in long-range contacts increases the...
Topics
- Bacteriophage T4
- Binding Sites
- Databases, Protein
- Enzyme Stability
- Models, Molecular
- Muramidase
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- Thermodynamics