Article
Building a replisome solution structure by elucidation of protein-protein interactions in the bacteriophage T4 DNA polymerase holoenzyme.
The Journal of biological chemistry - 19 Oct 2001
Alley S C, Trakselis M A, Mayer M U, Ishmael F T, Jones A D, Benkovic S J
Abstract excerpt
Assembly of DNA replication systems requires the coordinated actions of many proteins. The multiprotein complexes formed as intermediates on the pathway to the final DNA polymerase holoenzyme have been shown to have distinct structures relative to the ground-state structures of the individual proteins. By using a variety of solution-phase techniques, we have elucidated additional information about the solution...
Topics
- Biotin
- Calorimetry
- Chromatography, High Pressure Liquid
- Crystallography, X-Ray
- DNA
- DNA Replication
- DNA-Directed DNA Polymerase
- Holoenzymes
- Luminescent Measurements
- Mass Spectrometry
- Models, Molecular
- Mutation
- Protein Binding