Article
Construction of an in vivo nonsense readthrough assay system and functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus subtilis.
Journal of bacteriology - 1 Sept 2001
Inaoka T, Kasai K, Ochi K
Abstract excerpt
To investigate the function of ribosomal proteins and translational factors in Bacillus subtilis, we developed an in vivo assay system to measure the level of nonsense readthrough by utilizing the LacZ-LacI system. Using the in vivo nonsense readthrough assay system which we developed, together with an in vitro poly(U)-directed cell-free translation assay system, we compared the processibility and translational...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
