Article
A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity.
RNA (New York, N.Y.) - 1 Dec 2006
Kirthi Narayanaswamy, Roy-Chaudhuri Biswajoy, Kelley Teresa, Culver Gloria M
Abstract excerpt
S5 is a small subunit ribosomal protein (r-protein) linked to the functional center of the 30S ribosomal subunit. In this study we have identified a unique amino acid mutation in Escherichia coli S5 that produces spectinomycin-resistance and cold sensitivity. This mutation significantly alters cell growth, folding of 16S ribosomal RNA, and translational fidelity. While translation initiation is not affected, both...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Drug Resistance, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Glycine
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Ribosomal, 16S