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Article

Enrichment of mutant KRAS alleles in pancreatic juice by subtractive iterative polymerase chain reaction.

Laboratory investigation; a journal of technical methods and pathology - 1 Jun 2001

Fischer C, Büthe J, Nollau P, Hollerbach S, Schulmann K, Schmiegel W, Wagener C, Tschentscher P

Abstract excerpt

SUMMARY: The detection of mutant tumor genes holds great promise for an early diagnosis of primary tumors and residual malignant disease. When few tumor cells are present with an excess of nonmalignant cells of the same lineage, the excess of wild-type alleles over mutant tumor alleles presents an analytical problem. The subtractive iterative PCR (siPCR) assay presents a new approach to solving this problem. To...

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Identifiers

PMID: 11406644
DOI: 10.1038/labinvest.3780292

Genes

KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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