Article
Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilus.
Journal of molecular biology - 1 Jun 2001
Gregory S T, Cate J H, Dahlberg A E
Abstract excerpt
We have isolated spontaneous streptomycin-resistant, streptomycin-dependent and streptomycin-pseudo-dependent mutants of the thermophilic bacterium Thermus thermophilus IB-21. All mutant phenotypes were found to result from single amino acid substitutions located in the rpsL gene encoding ribosomal protein S12. Spontaneous suppressors of streptomycin dependence were also readily isolated. Thermus rpsL mutations...
Topics
- Alleles
- Amino Acid Sequence
- Bacterial Proteins
- Crystallography, X-Ray
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Models, Molecular
- Molecular Sequence Data
- Mutation, Missense
- Protein Conformation
- Protein Subunits
- Ribosomal Protein S9
- Ribosomal Proteins
- Ribosomes
- Sequence Alignment
- Streptomycin
- Suppression, Genetic