Article
Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution.
Protein engineering - 1 Mar 2001
Saab-Rincón G, Juárez V R, Osuna J, Sánchez F, Soberón X
Abstract excerpt
A monomeric version of triosephosphate isomerase from Trypanosoma brucei, MonoTIM, has very low activity, and the same is true for all of the additional monomeric variants so far constructed. Here, we subjected MonoTIM to directed evolution schemes to achieve an activity improvement. The construction of a suitable strain for genetic selection provided an effective way to obtain active catalysts from a diverse...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Amino Acids
- Animals
- Binding Sites
- Catalysis
- DNA Ligases
- Directed Molecular Evolution
- Enzyme Activation
- Escherichia coli
- Gene Library
- Genetic Variation
- Growth
- Kinetics