Article
Binding of double-stranded RNA to protein kinase PKR is required for dimerization and promotes critical autophosphorylation events in the activation loop.
The Journal of biological chemistry - 6 Jul 2001
Zhang F, Romano P R, Nagamura-Inoue T, Tian B, Dever T E, Mathews M B, Ozato K, Hinnebusch A G
Abstract excerpt
Protein kinase PKR is activated by double-stranded RNA (dsRNA) and phosphorylates translation initiation factor 2alpha to inhibit protein synthesis in virus-infected mammalian cells. PKR contains two dsRNA binding motifs (DRBMs I and II) required for activation by dsRNA. There is strong evidence that PKR activation requires dimerization, but the role of dsRNA in dimer formation is controversial. By making alanine...
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