Article
Requirement of PKR dimerization mediated by specific hydrophobic residues for its activation by double-stranded RNA and its antigrowth effects in yeast.
Molecular and cellular biology - 1 Dec 1998
Patel R C, Sen G C
Abstract excerpt
The roles of protein dimerization and double-stranded RNA (dsRNA) binding in the biochemical and cellular activities of PKR, the dsRNA-dependent protein kinase, were investigated. We have previously shown that both properties of the protein are mediated by the same domain. Here we show that dimer...
Topics
- Animals
- COS Cells
- Cell Division
- Cross-Linking Reagents
- Dimerization
- Enzyme Activation
- Heparin
- Mutation
- Protein Binding
- Protein Structure, Secondary
- RNA, Double-Stranded
- RNA-Binding Proteins
- Saccharomyces cerevisiae
- Transfection
- eIF-2 Kinase