Article
Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles.
PloS one - 1 Jan 2013
Ng Yen Kuan, Ehsaan Muhammad, Philip Sheryl, Collery Mark M, Janoir Clare, Collignon Anne, Cartman Stephen T, Minton Nigel P
Abstract excerpt
Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an efficient procedure for making precise alterations to the C. difficile genome by pyrE-based allelic exchange. The robustness and reliability of the method was...
Topics
- Alleles
- Bacterial Proteins
- Clostridioides difficile
- DNA, Bacterial
- Genetic Complementation Test
- Genetic Vectors
- Genome, Bacterial
- Phenotype
- Sequence Deletion